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Fig. 5. Presomitic mesoderm explants form VEGF-dependent vascular networks. 8.5 dpc
Flt1+/–, eGFP+/– mouse
presomitic mesoderm explants were embedded in collagen gels and cultured for
72 hours. Explants were stained for ß-gal activity (A,C,E), and the same
explants were visualized for eGFP expression (B,D,F). Explants were incubated
with basal medium alone (A,B), or with added 30 ng/ml VEGFA (C,D) or 25 ng/ml
bFGF (E,F). n
7 for all three conditions. The arrows in C indicate
the vascular plexus, and the arrowheads in A and E indicate ß-gal
positive (blue) presumptive vascular cells. Scale bar: 200 µm for all
panels.
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