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Fig. 6. Neural tube-dependent vascular plexus formation in presomitic mesoderm
explants. Flt1+/– mouse presomitic mesoderm grafts
(PSM) (8.5 dpc) were co-cultured with HH10-13 stage quail neural tubes (NT) or
notochords (NC) in collagen gels for 72 hours. Individual co-cultures were
photographed at 0 (A,D), 24 (B,E) or 48 hours (C,F) to demonstrate the growth
of the tissues. The same cultures were then fixed at 72 hours and stained for
ß-gal (G,H). The purple arrows indicate the respective tissues and the
black arrows indicate the vascular plexus that forms in PSM/NT co-cultures.
The arrowheads indicate the few ß-gal positive presumptive vascular cells
seen in PSM/NC co-cultures. n>10 for PSM/NT co-cultures and
n=3 for PSM/NC co-cultures. Scale bars: 350 µm in A-F; 140 µm
in G,H.
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