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Fig. 8. A genetic mutation in the VEGF receptor Flk1 blocks neural
tube-derived vascular plexus formation. Mouse presomitic mesoderm explants
were cultured for 72 hours in basal medium with either 30 ng/ml VEGFA (A,B) or
stage HH10-13 quail neural tubes (C,D). Explants were either
Flk1+/– (A,C) or
Flk1–/– (B,D), and mouse vascular cells were
visualized by ß-gal staining. The arrows (A,C) indicate mouse vascular
plexus, and the arrowheads (B,D) point to ß-gal positive (blue)
presumptive mouse vascular cells. n
7 for all conditions. Scale
bar: 200 µm for all panels.
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