QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 1. Oncopeltus embryogenesis and engrailed expression. (A,B) Blastoderms stained with SYTOX to show nuclei. Anteroposterior axis and dorsoventral axis are indicated. (A) Early blastoderm at 24-28 hours. (B) Late blastoderm at 36-40 hours. Arrowhead indicates site of germband invagination. Scale bar: 200 µm. (C) Late blastoderm at 36-40 hours hybridized with engrailed probe (purple color). The six en stripes are labeled: MN, mandible; MX, maxillary; LB, labium; T1, first thoracic; T2, second thoracic; T3, third thoracic. Arrowhead indicates site of germband invagination. (D1) Embryo stained for engrailed at slightly later stage than in C. Note that only the four anterior en stripes are now visible on the blastoderm surface. (D2) Same embryo as in D1, with `dorsal' region of blastoderm dissected away to reveal developing germband and rotated to view dorsal aspect. Note that the en stripes corresponding to the second and third thoracic segments are now on the invaginated germband. (E-H) Dissected germbands stained for engrailed, showing growth of the posterior and the sequential addition of the abdominal segments. Anterior is towards the top. (E) Early germband just completing germband invagination. (F) Forty-four- to 48-hour-old germband. A1, first abdominal engrailed stripe; GZ, posterior growth zone. (G) Embryo with five abdominal segments. (H) Fully elongated germband with the tenth and final abdominal engrailed stripe. Scale bar: 100 µm in F,H.

Return to article