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Fig. 2. Analysis of the Oncopeltus fasicatus hunchback sequence. (A)
Predicted Oncopeltus fasciatus hunchback (O.f.) zinc-finger
domains aligned with homologous domains from Schistocerca americana
(S.a.), Drosophila melanogaster (D.m.), and
Tribolium castaneum (T.c.). NF-1 and NF-2 are the
amino-terminal two zinc fingers, MF 1-4 are the middle four fingers, and CF-1
and CF-2 are the carboxy fingers. Arrowheads indicate the locations of the
critical cysteine and histidine residues. (B) Alignments of the
Oncopeltus A-, Basic, and C-boxes with other insect species. (C) The
predicted Oncopeltus huncback structure showing the locations of the
zinc fingers (black boxes) and other conserved motifs (gray boxes). The
locations of the 300 bp, 500 bp, and 1.1 kb dsRNA fragments used in RNAi are
mapped above the protein structure. An asterisk indicates the second 300 bp
fragment. (D) The predicted structures of insect hunchback proteins
mapped upon the insect phylogeny. As both Schistocerca and
Oncopeltus hunchback proteins are predicted to contain a total of
eight zinc fingers, this is probably the ancestral state for the eumetabola
(arrowhead). As Tribolium and Drosophila hunchback have only
six zinc fingers, hunchback appears to have lost its two N-terminal
fingers in the lineage leading to holometabola.
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