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Fig. 4. hunchback blastoderm in situs. Anterior is towards the left in all
images. (A) Twelve- to 16-hour-old blastoderm. hb transcript is
diffuse along length of egg but is concentrated in the central region of the
embryo (arrowheads). (B) Twenty- to 24-hour-old blastoderm. Accumulation in
the central region of blastoderm becomes more pronounced. (C1-C2)
Twenty-eight- to 32-hour-old embryo. (C1) Ventral view. Two bands of
hb accumulation are apparent. Anterior and central bands (white and
black arrowheads, respectively, are indicated. Posterior hb patch is
indicated by an arrow. (C2) Dorsal view of blastoderm. hb expression
is weaker in dorsal region of blastoderm. (D1-D2) Thirty-six- to -40-hour-old
embryo. Bands of expression are fading from blastoderm surface and the
appearance of hb expression in the underlying yolk nuclei. (D1)
Lateral view. Arrow indicates the beginnings of germband invagination. (D2)
Dorsal view. (E) Composite image consisting of two 36- to 40-hour-old embryos
stained for engrailed (top) and hunchback (bottom). The
central band of hunchback expression spans the first three
engrailed stripes that correspond to the posterior of the mandibular,
maxillary, labial and anterior of the first thoracic segments. Scale bar: 200
µm.
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