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Fig. 5. hunchback transcript expression in germband embryos. Anterior is upwards in all images. (A) Very early germband undergoing invagination. At this stage, tissues fated to become head have not yet invaginated, and are therefore not represented in this early germband preparation. hb transcript in blocky chevron pattern in the early thorax. (B) Germband invagination complete. Labial (LB) and third thoracic segment (T3) are labeled. Note that hb does not accumulate in a gap-like pattern in the labium, but rather in a segmentally reiterated blocky chevron pattern. (C-F2) Germbands double stained for hunchback (purple) and engrailed (orange). (C) Germband beginning elongation. Labial (LB) and first abdominal (A1) segments are labeled. Arrows indicate two dots of hb expression in the posterior zone. (D) Slightly later embryo than in C, posterior dots of hb have now expanded to become a half-moon patch in the posterior (arrow). (E) hunchback expression persists throughout germband extension. The sixth abdominal segment (A6) is labeled. Also note that hunchback is now expressed in a neural pattern in the trunk of the germband. (F1) hunchback is expressed in the posterior until the formation of the tenth abdominal segment (arrowhead). (F2) Close up of posterior germband in (F1). Scale bars: 200 µm in A-F1.





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