|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 7. Class I-III (mild and moderate) hunchback RNAi phenotypes. (A1-A3)
Uninjected embryos. Anterior towards the left. Thoracic and abdominal regions
are delineated. (A1) Lateral view. (A2) Dorsal view. (A3) Ventral view.
Arrowhead indicates the mandibular stylet, arrow indicates the maxillary
stylet. AN, antenna; LB, labium; T1, first thoracic leg; T2, second thoracic
leg; T3, third thoracic leg. (B1-B2) Class I embryo. (B1) Lateral view. (B2)
Ventral view. Labium is a lump of undifferentiated tissue. T1 leg is reduced,
while T2 and T3 legs are less strongly affected. (C1,C2,C4,C5) Class II
embryo. (C1) Lateral view. Abdominal region indicated. (C2) Close-up of
gnathal and anterior thorax of embryo in C1. Arrowheads indicate
abdominal-like spiracles. (C3) Close-up of abdominal spiracles (arrowheads) on
uninjected animal. (C4) Ventral view of embryo shown in C1, C2. Reduced second
and third thoracic legs are labeled. (C5) Close-up of gnathal region of embryo
shown in C4. Arrowhead indicates location of ventral fourth abdominal (A4)
spiracle. (C6) Close up of A4 of uninjected animal. Spiracle indicated with
arrowhead. Scale bar: 50 µm. (D1-D2) Class III embryo. (D1) Dorsal view.
Note dorsal segmentation defect. Abdominal region indicated. (D2) Ventral
view. Defective third thoracic leg (T3) is labeled. Note abdominal-like
segmentation in gnathal region of animal. (E) Wild-type germband stained for
abdominal-A. Note expression in second through eighth abdominal
segments and lack of expression in labial segment. (F) Putative class I embryo
stained for abd-A. Note reduced labium (LB). (G) Putative class III
embryo stained for abd-A. (H) Putative class II embryo stained for
engrailed. Scale bars: 200 µm in A-C1,C4,D; 50 µm in C6; 100
µm in E-H.
|
