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Fig. 8. Class IV and V embryos. (A) Dorsal view of class IV embryo. This embryo did
not complete dorsal closure. Note defective segmentation and shortened
abdominal region. (B) Class IV embryo. Arrow indicates posterior leg. (C1)
Class IV/V embryo. Lateral view. Arrow indicates posterior leg. (C2) Dorsal
view of embryo in panel C1. Note shortened abdominal region. (D) Dorsal view
of class IV/V embryo. Note extremely shortened abdominal region. Normal
antenna (AN) is indicated. (E) Class V embryo. (F) Putative class IV embryo
stained for engrailed. Third thoracic leg is indicated (T3). Note
highly reduced labium and T1 and T2 legs and defective segmentation in
abdomen. Note that segmentation in labium (LB) and thoracic region (T1-T3)
appears normal. (G) Putative class IV/V embryo stained for en. T3 leg
reduced. Note defective segmentation in abdomen and normal segmentation in
labium (LB) and thoracic region (T1-T3). (H) Putative class V embryo stained
for abd-A. abd-A expression extends from the labial segment to the
posterior of the animal. Maxillary (MX), mandibular (MN) and labial (LB)
segments appear relatively normal. Scale bars: 200 µm in A,E; 100 µm. in
F-H.
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