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Fig. 7. Control (A-L) or morpholino-injected (M-
) embryos hybridised with
probes against deltaC (A-K,M-T',Y-ß) or her1
(I-L; U-X;
,
) at different time points. (A-L) In control
embryos, stripes of deltaC and her1 are maintained
throughout somitogenesis. (D',D'') Higher magnifications of the
boxed areas in the anterior and posterior PSM, respectively, of the embryo in
D. (I') High magnification of the boxed area in I. (M-X) In embryos
injected with 3 ng her6MO+3 ng her4MO, the periodicity of deltaC and
her1 expression breaks down between 8 and 10 ss and is lost by 14 ss.
deltaC stripes become blurred between 8 and 10 ss (M-O). Between 10
and 14 ss, the anterior stripes fuse (P,Q), lose their homogeneous character
(compare R', a magnification of the boxed area in R, with D') and
are replaced by an irregular domain expressing deltaC at different
levels (S,S',T,T'). (S',T') Higher magnifications of
the boxed areas in the anterior PSM of S and T. (S'') Higher
magnification of the posterior boxed area in S, showing that an irregular
deltaC pattern is also found in the posterior PSM (compare S''
with D''). her1 stripes become blurred around 10 ss (compare
V,V' with I,I') and by 14 ss a mixture of cells expressing
her1 at different levels is found throughout the PSM (W-X).
(Y-
) In embryos injected with 6 ng her6MO the periodicity of
deltaC expression is maintained at 14 ss (Y), breaks down around 20
ss (Z) and is lost by 26 ss (
). (
,
) disruption of her1
starts around 18 ss and is complete by 26 ss. (ß) In an embryo injected
with 3 ng her6MO, only weak deltaC abnormalities are visible at 26
ss.