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Fig. 3. Mos is able to activate MAPK and Rsk in stage IV oocytes but does not promote MPF activation. Stage IV and stage VI oocytes were injected with mouse Mos mRNA. After overnight incubation, they were incubated or not in the presence of progesterone. Stage IV oocytes were collected 4 hours after GVBD occurred in stage VI oocytes. Oocyte extracts were western blotted with antibodies against Rsk, Cdc25, Xenopus Mos (XeMos), the active phosphorylated form of MAPK (P-MAPK), the Tyr15-phosphorylated form of Cdc2 (P-Tyr-Cdc2), cyclin B1 and cyclin B2.





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