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Fig. 4. Okadaic acid is unable to induce MPF auto-amplification in stage IV oocyte
extracts. Stage IV and stage VI oocyte extracts were incubated in the presence
(+) or in the absence (–) of 1 µM okadaic acid (OA) and an
ATP-regenerating system for 3 hours. (A) Histone H1 kinase assay of Cdc2. (B)
Western blots revealed with antibodies against the active phosphorylated form
of MAPK (P-MAPK), Cdc25 and the Tyr15-phosphorylated form of Cdc2
(P-Tyr-Cdc2). (C) Oocyte extracts were supplemented with
[35S]methionine labeled Myt1 at time of OA addition. Extracts were
then submitted to SDS-PAGE and to autoradiography.
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