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Fig. 6. Misexpression of Zic3 did not alter the expression of islet 1 or induce the
expression of a ciliary margin marker, collagen IX. RCAS-Zic3 virus-infected
retinas were analyzed at E7. Samples were double stained with an anti-viral
GAG antibody (B,F) and an anti-islet 1 antibody (A), or an anti-collagen IX
antibody 2C2 (C,E). (D) A bright-field image is shown. The same areas are
shown in A and B, C and D, and E and F, respectively. Note that there was no
apparent difference in either the number or distribution of islet 1-expressing
cells in the infected area (A,B) compared with the uninfected wild-type area.
Collagen IX is highly expressed in the very periphery of the retina, the
prospective ciliary margin region (arrow in C), but not inside the retina,
identical to the patterns in the uninjected control retina (not shown). Scale
bar: 50 µm.
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