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Fig. 7. Misexpression of Zic3 did not grossly alter retinal lamination.
Cryosections of E10 retinas infected with RCAS-Zic3 were stained with
anti-neurofilament (A-C) and various cell type-specific antibodies including:
anti-visinin (G), anti-islet 1 (H) and anti-Pax6 (I). (C-F) Same
RCAS-Zic3-infected retinal section was shown stained with anti-neurofilament
(C), DAPI (D), anti-viral GAG antibody (F) and in phase contrast (E). Note
some axons in the RCAS-Zic3-infected retinas misrouted to the subretinal
spaces at the photoreceptor side of the retina (arrowheads, A-C). However, the
laminated structure of the retina was largely normal (D-I), except some
dispersion of cells towards the photoreceptor side (arrowheads, H and I). The
optic fiber layer was largely normal, except for some protrusions of the axons
(arrows in A,B). Frequently, however, the photoreceptor layer was broken
(arrowheads, D and G). Note that the anti-viral GAG antibody staining was much
more intense at the sites of axon crossing-over than the nearby infected
region (arrowhead in F). PR, photoreceptor layer; OFL, optic fiber layer; GCL,
ganglion cell layer; INL, inner nuclear layer.
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