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Fig. 9. Stripe assays. The membrane fragments prepared from various retinal tissues
were compared for their abilities to support axonal growth by stripe assays.
Green fluorescent beads were mixed in to mark the second stripes. (A) The
membrane fragments prepared from the central halves of the uninjected retinas
were laid down both in the first and second stripes. (B) The membrane
fragments prepared from the RCAS-Zic3-injected retinas were laid down in the
first stripes, whereas those from the RCAS-GFP-injected retinas were filled in
as the second stripes. (C) Membrane fragments prepared from the peripheral E8
retinas were laid down in the first stripes, whereas those from the central E8
retinas were filled in as the second stripes. Note that the membrane fragments
prepared from the RCAS-GFP-injected retina were more preferable than those
from the RCAS-Zic3-injected retina, and the membrane preps from the central
retinas were more preferable than those from the peripheral retinas. (D)
Quantification of the stripe assay results. Scale bar: 100 µm.
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