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Fig. 1. Identification of botv and sotv as two novel segment-polarity genes. (A-D) Wings are oriented proximal to the left, anterior up. (A) A wild-type wing. (B-D) Wings bearing somatic clones of ttv⁶³ (B), botv¹⁰³ (C) and sotv⁴⁴ (D). Adult wings with clones of these mutations exhibit a variety of phenotypes, including vein loss, vein fusion, blister, narrowed wing and wing notching (data not shown). (E-I) Cuticle preparations of a wild-type embryo (E), a hh^IJ35 homozygous embryo (F), and embryos derived from mutant germ-line clones of botv¹⁰³ (G) and sotv⁴⁴ (H,I). All embryos are oriented anterior to the left. Embryos lacking both maternal and zygotic activities of botv or sotv exhibit typical segment-polarity phenotypes; however, the defects of sotv mutants can be relatively weak (I). (J-L) En staining of a stage 10 wild-type embryo (J), and embryos derived from mutant germ-line clones of botv¹⁰³ (K) and sotv⁴⁴ (L). (M-O) Wg staining of a stage 10 wild-type embryo (M), and embryos derived from mutant germ-line clones of botv¹⁰³ (N) and sotv⁴⁴ (O).

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