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Fig. 3. The Drosophila EXT proteins are required for the biosynthesis of HS GAG chains in vivo. Third instar larval wing imaginal discs carrying mutant clones of ttv63 (A,A'), sotv44 (B,B') and botv103 (C,C') were fixed, digested with bacterial heparinase III and then stained with mAb 3G10, which recognizes the epitope generated by heparinase III digestion. The mutant clones are marked by the absence of GFP and are outlined with dots. 3G10 staining is absent in mutant clones of ttv63, sotv44 and botv103.





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