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Fig. 6. Involvement of the Drosophila EXT genes in Wg signalling. In all discs carrying mutant clones, the clones are marked by the absence of GFP and are outlined with dots. (A-D'') Dll and Sens staining in a wild-type wing disc (A-A''), and in wing discs carrying mutant clones of ttv⁶³ (B-B''), sotv⁴⁴ (C-C'') and botv¹⁰³ (D-D''). Dll expression appears to be a gradient with the peak at the DV boundary. The ranges of the Dll gradient in the mutant clones of ttv⁶³ (B), sotv⁴⁴ (C) and botv¹⁰³ (D) are reduced. Note that the level of Dll expression close to the DV boundary within the mutant clones of ttv⁶³ and sotv⁴⁴ is comparable with that of wild-type cells; however, it is significantly reduced in the botv¹⁰³ mutant clone. Wg-dependent Sens expression in the wing disc is in two narrow stripes abutting Wg expressing cells. This expression is unaffected in the mutant clones of ttv⁶³ (B') and sotv⁴⁴ (C'), but is diminished in the botv¹⁰³ mutant clone (D'). Note that the Wg-independent expression of Sens in botv¹⁰³ mutant clone is still maintained. (E-H') Extracellular Wg distribution is shown in a wild-type wing disc (E), and in discs carrying mutant clones of ttv⁶³ (F,F'), sotv⁴⁴ (G,G') and botv¹⁰³ (H,H'). Extracellular Wg is almost completely lost in the botv¹⁰³ clone except at the surface of Wg expressing cells, whereas it is maintained at some level around the DV boundary in the mutant clones of ttv⁶³ and sotv⁴⁴. (I-J') Sens staining in discs bearing clones of ttv⁶³-sotv⁴⁴ double mutant cells. The Wg-dependent Sens expression is diminished in the double mutant clones.

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