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Fig. 2. PTX3 is expressed by cumulus cells in the preovulatory follicle and localizes in the matrix. (A) In situ hybridization of an ovary isolated from a naturally cycling mouse. (B) Kinetics of Ptx3 mRNA expression in ovaries during hormonally induced ovulation. Each lane was loaded with 10 µg of total RNA. Ethidium bromide staining of the gel is shown in the lower panel. (C) Detection of PTX3 protein in ovulated intact cumuli oophori (COC), isolated cumulus cells (CC), denuded oocytes (O) and cumulus matrix (matrix) assessed by western blotting. (D) Immunofluorescence analysis (left panel) and phase contrast (right panel) of a cumulus oophorus. Scale bars: 100 µm in A; 50 µm in D.





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