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Fig. 2. Expression of members of the Notch signalling pathway during proventriculus
development. (A-D) Dl expression in the ectodermal cells of the
keyhole monitored by Anti-Dl (red)/anti-Dve (green) immunostaining at stage 12
(A), stage 15 (B), stage 16 (C) and late stage 17 (D). Delta localises to the
ectodermal keyhole domain which moves into the endodermal cell layer. Arrows
(C,D) mark the downregulation of Dl expression in anterior (ac) and posterior
(pc) boundary cells. (E-L) Dynamic Notch receptor expression visualised in a
single channel visualisation (E-H) and in an anti-Notch (red)/anti-Dve (green)
immunostaining (I-L). (E,I) Stage 13; (F,J) stage 14; (G,K) stage 15; (H,L)
late stage 17 wild-type embryos. The Notch receptor is upregulated in ac and
pc. (M-P) Relative localisation Delta (green) and Notch signalling activity
[Gbe-Su(H)m8-lacZ; red] during proventriculus development.
The epithelial gut tube is surrounded by broken lines; ac and pc are
highlighted by arrows. Notch signalling activity is restricted to the ac and
pc, which are adjacent to the Delta expression domain.
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