spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 1. (A-M) Programmed cell death of wing epidermal cells in en-Gal4 UAS-GFPN flies. Wing epidermal cells were visualized by nuclear-localized GFP. GFP was apparent in wing epidermal cells of the posterior compartment at 0 hours (A) and 0.5 hours (B) after eclosion, but disappeared by 2 hours after eclosion (C). Higher magnification images of cell death at 0 minutes (D), 10 minutes (E), 20 minutes (F), 30 minutes (G), 40 minutes (H) and 60 minutes (I) after wing spreading. At wing spreading, wing epidermal nuclei are labeled by GFP (D). The GFP signal was then dispersed into the cytoplasm from the nucleus following breakdown of the nuclear membrane (E,F; arrows). GFP was undetectable in the nuclei of all cells after 30 minutes (G), and the cells were detached from the wing cuticle and floated in the cavity formed by the dorsal and ventral wing surfaces (H). After 60 minutes, the wing epidermal cells had disappeared and GFP was detectable only in the nuclei of vein cells (I, arrow). At wing spreading, GFP in the nuclei of wing epidermal cells (J) corresponded to nuclei stained with DAPI (K). At 20 minutes after wing spreading, GFP was dispersed in the cytoplasm (L, arrows). DAPI staining showed that the chromatin of these cells was fragmented (M, arrows). (N-Q) Transverse sections of adult wings in wild-type flies. At eclosion, two layers of wing epidermal cells were stained with DAPI (N) but were not labeled with TUNEL (O). At 20 minutes after wing spreading, the wing epidermal cells were stained with DAPI (P) and were labeled with TUNEL (Q), except for vein cells (arrows; P,Q). Transmission electron microscopy in a wing epidermal cell at 0 hours after eclosion (R) and at 20 minutes after wing spreading (S). The arrow (S) indicates representative condensed chromatin structures. Arrowheads (S) denote cytoplasmic vacuoles that contain cellular structure including mitochondria. Scale bars: 1 µm in R,S. Ectopic expression of p35 inhibited the death of wing epidermal cells in en-Gal4 UAS-GFPN /UAS-p35 flies at 2 hours (T) or even at 48 hours after eclosion (U).





Right arrow Return to article