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Fig. 2. Effects of neck ligation or wing isolation on the death of wing epidermal cells in en-Gal4 UAS-GFPN flies. Induction of cell death was examined at 2 hours after neck ligation (A-C) or wing isolation (D-F). Neck ligation or wing isolation in flies just after eclosion suppressed cell death (A,D, respectively), but treatment at 20 minutes after eclosion did not prevent the death (B,E). The percentage of flies showing wing epidermal cell death increased with time of treatment (C,F). Black and gray bars indicate the percentage of flies showing cell death extensively or in restricted domains of the wing, respectively (see Materials and methods). The number of flies examined is shown in parentheses.





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