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Fig. 4. Effects of 8-Br-cAMP injection on the death of wing epidermal cells in
en-Gal4 UAS-GFPN flies neck-ligated at eclosion. Cell death was
induced by injection of 8-Br-cAMP at a concentration of 10–1
mol/l (A) but not by injection of PBS (B). Injection of 8-Br-cAMP produced a
dose-dependent effect on the induction of cell death (C). 8-Br-cAMP
(10–1 mol/l) was injected into pharate adult flies
neck-ligated at various stages and wings were examined for cell death 2 hours
after injection (D). (C,D) Black and gray bars indicate the percentage of
flies showing cell death extensively or in restricted domains of the wing,
respectively. The number of flies examined is shown in parentheses. 8-Br-cAMP
(10–1 mol/l) was injected into intact flies at EP stage (just
before eclosion). A control fly injected with PBS spread its wings normally
(E). A fly injected with 8-Br-cAMP had blistered wings (F, arrow), in which
the epidermal cells had died prior to spreading (G). Arrows (G) indicate the
persistence of GFP in vein cells.
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