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Fig. 5. Effects of ectopic expression of the wild-type Gs
subunit in
en-Gal4 UAS-GFPN / +; UAS-Gs/+ flies (A), or a
constitutively active form of Gs (Gs*) in en-Gal4 UAS-GFPN /
+; UAS-Gs*/+ flies (B,C). Ectopic expression of wild-type
Gs produced no visible phenotype (A), whereas Gs* caused wing
blisters (B, arrow). Precocious cell death had occurred in blistered wings at
the time of wing spreading (C). The arrow (C) indicates GFP remaining in the
vein cells. The onset of precocious cell death induced by ectopic expression
of Gs* was examined at various stages in pharate adults (D). Black and
gray bars indicate the percentage of flies showing cell death extensively or
in restricted domains of the wing, respectively. The number of flies examined
is shown in parentheses. Effects of elimination of Gs activity on the
cell death of wing epidermal cells (E,F). Mutant clones of dgs were
marked by the sha– phenotype of missing or smaller
hairs (E, enclosed by white lines). The cells of the clones remained at 2
hours after wing spreading, although neighboring cells had died (F). The arrow
(F) indicates the persistence of GFP in anterior wing margin cells.
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