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Fig. 1. Expression of Barhl2 during CNS development. (A-P) The
Barhl2 transcript was detected by in situ hybridization in
whole-mount embryos (A,B) and in sagittal (C) and coronal (D-P) sections from
embryonic and postnatal mice at the indicated developmental stages.
Barhl2 expression is initiated at E9.5 in two symmetric stripes
within the diencephalon (A). At later stages, its expression is found in
select regions of the telencephalon, diencephalon, mesencephalon, cerebellum,
medulla and spinal cord (B-P), as well as in the retina and pineal gland
(E,F). aes, anterior extramural migratory stream; AP, alar plate; BP, basal
plate; Cb, cerebellum; D, diencephalon; D1, D1 interneuron; DB, diagonal band;
EGL, external granule layer; ET, epithalamic neuroepithelium; IC, inferior
colliculus; ION, inferior olivary nucleus; IT, intermediate thalamic
neuroepithelium; LRL, lower rhombic lip; M, mesencephalon; Me, medulla; MM,
mammillary region; NR, neural retina; NT, neural tube; PA, periaqueductal
gray; PC, pyriform cortex; PG, pineal gland; PGN, pontine gray nucleus; PO,
preoptic area; PV, paraventricular nucleus; SC, superior colliculus; SpC,
spinal cord; ST, spinal trigeminal nucleus; T, telencephalon; URL, upper
rhombic lip; VT, ventral thalamus. Scale bar: 25 µm in I; 50 µm in C,O;
100 µm in D,P; 250 µm in A,E-N; 333 µm in B.
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