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Fig. 4. Effect of forced Barhl2 expression on retinal inner nuclear cells. (A)
Schematic of control-GFP and Barhl2-GFP retroviral constructs. In Barhl2-GFP,
a bicistronic transcript containing an internal ribosomal entry site (IRES) is
produced from the viral LTR promoter, which allows efficient expression of
both Barhl2 and the GFP marker protein. (B,C) Virus-transduced GFP+
(green) cells were visualized in P30 retinal sections that were weakly
counterstained with nuclear DAPI. Infection with Barhl2-GFP viruses caused a
significant increase of cells situated within the inner region of the inner
nuclear layer. Arrows in C indicate two such cells. (D) Percentages of
virus-transduced cells located in different cellular layers of the retina
(means±s.d.). More than 600 GFP+ cells in three independent
retinas were scored for either control-GFP or Barhl2-GFP virus. The inner
nuclear layer was divided into the outer and inner halves for quantitation.
GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform
layer; ONL, outer nuclear layer; OPL, outer plexiform layer. Scale bar: 10
µm.
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