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Fig. 5. Effect of misexpressed Barhl2 on the formation of different retinal cell types. (A-P) Sections from P30 retinas infected with control-GFP (A,C,E,G,I,K,M,O) or Barhl2-GFP (B,D,F,H,J,L,N,P) viruses were double-immunostained with an anti-GFP antibody and antibodies against syntaxin (A,B), Pax6 (C,D), glycine transporter 1 (GLYT1) (E,F), calbindin (G,H), GABA (I,J), PKC ${alpha}$ (K,L), glutamine synthase (GS) (M,N) or Brn3a (O,P). All sections were weakly counterstained with nuclear DAPI. Forced Barhl2 expression resulted in a significant increase in the number of amacrine cells immunoreactive for syntaxin (A,B), Pax6 (C,D), GLYT1 (C,D) and calbindin (E,F), whereas it had no effect on amacrine cells immunoreactive for GABA (I,J) and ganglion cells immunoreactive for Brn3a (O,P). Moreover, it greatly suppressed the formation of rod bipolar cells immunoreactive for PKC ${alpha}$ (K,L) and Müller cells immunoreactive for GS (M,N). Arrows indicate representative colocalized cells. GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer. Scale bar: 25 µm.

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