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Fig. 7. Effect of a dominant-negative form of Barhl2 on the formation of different
retinal cell types. (A) Schematic of control-GFP and Barhl2-EnR-GFP retroviral
constructs. (B) Percentages of virus-transduced cells located in different
cellular layers of the retina (means±s.d.). More than 500
GFP+ cells in three or four independent retinas were scored for
either control-GFP or Barhl2-EnR-GFP virus. (C) Percentages of
virus-transduced cells that became immunoreactive for a series of cell
type-specific markers. Each histogram represents the mean±s.d. for
three to five retinas. More than 500 GFP+ cells were scored in each
retina. (D,E) Virus-transduced GFP+ cells were visualized in P30
retinal sections that were weakly counterstained with DAPI. Infection with
Barhl2-EnR-GFP viruses resulted in a significant decrease of INL cells. (F-K)
Sections from P30 retinas infected with control-GFP or Barhl2-EnR-GFP viruses
were double-immunostained with an anti-GFP antibody and antibodies against
Pax6 (F,G), GLYT1 (H,I) or GABA (J,K). All sections were weakly counterstained
with DAPI. Forced Barhl2-EnR expression reduced the number of amacrine cells
immunoreactive for Pax6 (F,G) and GLYT1 (H,I) but had no effect on amacrine
cells immunoreactive for GABA (J,K). Arrows indicate representative
colocalized cells. EnR, engrailed repressor domain; GCL, ganglion cell layer;
GS, glutamine synthase; INL, inner nuclear layer; IPL, inner plexiform layer;
ONL, outer nuclear layer; OPL, outer plexiform layer. Scale bar: 25 µm.
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