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Fig. 3. Basement membrane-like structures are deposited in the absence of collagen IV. Collagen IV (Col4), nidogen-1 (Nd-1) and laminins containing {alpha}1, ß1 or {gamma}1 subunits (Ln) were detected by immunohistochemistry at E10.5 in embryos (A) or in Reichert's membrane (B) with the indicated genotypes. Basement membrane discontinuities are indicated by arrows. N, notochord; P, pial basement membrane; E, epidermal basement membrane; RM, Reichert's membrane; T, trophoblast cells. Scale bars: 100 µm.





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