|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Deficiency of 
1(IV)/2(IV) collagen is not compensated by the
increased expression of other collagen IV chains. (A) Relative mRNA levels of
collagen IV isoforms (3-6), laminin 1 chain (Ln-1)
and nidogen-1 (Nd-1) were determined by quantitative RT-PCR in RNA isolated
from individual wild-type (white bars) and 1(IV)/2(IV)-deficient
embryos (black bars) from E9.5 and E10.5 littermates. The 3(IV),
4(IV) and 6(IV) mRNAs are not detectable or are present at only
very low levels in any genotype. In mutant embryos no compensatory
upregulation of 5(IV), laminin 1 chain and nidogen-1 is
observed. Relative mRNA levels are expressed as a percentage of GAPDH
expression. (B) No deposition of 5(IV) protein is observed by
immunofluorescence in the Reichert's membrane of normal (+/+) or mutant
embryos (–/–) at E10.5. A faint intracellular staining in
trophoblast cells indicates the expression of low levels of non-secreted
5(IV) protein. Antibodies detecting laminins containing the 1,
ß1 or 
1 chains and 2(IV) collagen were used as positive
controls. Scale bar: 10 µm.
|
