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Fig. 2. Mutations in the TRPV channel proteins do not cause general cell fate
transformation of the ADF neurons. (A) Expression of a ocr-2::gfp
reporter in a wild-type animal. Both osm-9 and ocr-2 are
expressed in ADF (Tobin et al.,
2002), but not in other serotonergic neurons. (B-D) Expression of
ADF markers in osm-9(yz6) and ocr-2(yz5) mutants. The GFP
reporters were examined in wild-type, yz6 and yz5 mutant
animals. The expression patterns in wild-type animals have been published:
lin-11:gfp (Hobert et al.,
1998), and cat-1::gfp and gtpch-1::gfp
(Sze et al., 2002).
Representative examples of the reporter expression patterns in mutant animals
are shown. (B) The expression of lin-11::gfp overlaps with
osm-9 and ocr-2 in the ADF and ADL chemosensory neurons.
Shown is a yz6 mutant animal strongly expressing lin-11::gfp
in ADF and ADL. The position of the cell body and the morphology of the axon
and dendrite are normal (indicated by arrowheads). (C) A GFP reporter of the
CAT-1/vesicular monoamine transporter is localized to the synapses of the
serotonergic neurons and dopaminergic neurons
(Sze et al., 2002). Unlike the
dramatic reduction of tph-1::gfp expression in the ADF neurons
(Fig. 1B,
Table 1), cat-1::gfp
can be clearly observed in the ADF neurons of yz5 and yz6
mutants. We have repeatedly observed that cat-1::gfp expression in
the ADF neurons is relatively weaker in yz5 and yz6 mutants
than in wild-type animals, but the differences were not statistically
significant. The GFP is distributed as punctate pattern, presumably the fusion
CAT-1 is associated with synaptic components. (D) A GFP reporter of a probable
GTP-cyclohydrolase I gene gtpch-1 is strongly expressed in
serotonergic and dopaminergic neurons (Sze
et al., 2002). Shown is a yz6 animal expressing
gtpch-1::gfp in ADF and in other serotonergic neurons.
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