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Fig. 3. TRPV channel-dependent regulation of tph-1 expression is mediated
by a specific cis-regulatory region of tph-1 and is modulated by
unc-43 CaMKII. (A) Expression of a GFP reporter under the control of
the sequence –132 to –377 of tph-1 and a pes-10
minimal promoter in wild-type and osm-9 mutant animals. In wild-type
animals, GFP is strongly expressed in the ADF neurons, but not in any other
serotonergic neuron, indicating that this tph-1 cis-regulatory region
specifically mediates tph-1 expression in the ADF neurons. The ADF
expression of this GFP reporter is significantly reduced in yz6
mutant animals; hence, the TRPV signaling stimulates this neuron-specific
transcriptional mechanism. In addition to ADF, this GFP reporter is often
expressed in two other neurons, which are tentatively identified as the ASI
chemosensory neurons based on the relative position of the cell body
(White et al., 1986). The GFP
intensity in ASI is not significantly affected by the TRPV mutation. (B)
tph-1::gfp expression is unaffected by G
mutations but is
modulated by unc-43 CaMKII activity. All the strains bear the same
GFP reporter. The average of ADF GFP intensity in wild-type animals is defined
as 1, and the average GFP intensity in other strains is normalized against the
wild-type average. The data are the summary of four independent trials, and
the total number of animals scored for each strain is indicated next to the
bar. Error bars indicate the mean of the standard error (s.e.m.).
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