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Fig. 4. Role of mesenchyme in the induction of uterine epithelial phenotype. ECM,
extracellular matrix. Expression of PR (left column) and ER
(right
column) in UtE was examined in vivo (A-D, I-L) and in vitro (E-H). In the E18
uterine anlage, both PR (A) and ER (B) were undetectable in the
epithelium. By P5, UtE in situ became strongly positive for PR (C) but was
negative for ER (D). By contrast, when isolated E18-UtE was cultured in
vitro for 7 days, PR was detected in only a subset (<20%) of epithelial
cells (E), while ER was detected in more than a half of epithelial
cells. By contrast, adult-UtE maintained a high level of PR (G) and ER
(H) under the same culture conditions. When E18-UtE was recombined with UtM,
the epithelium expressed PR (I) and ER (J) after 1 month of in vivo
growth. In the same host, E18-UtE recombined with BLM did not express PR (K),
while ER (L) was strongly expressed.
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