|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. Endoderm potential of GFP-Bry+ cells. (A) FACS profile of day
2.5 EBs and reaggregation and culture protocol for differentiation of the
sorted cells. (B) RT-PCR expression analysis of pre-sorted (p),
GFP-Bry– and GFP-Bry+ cells. d2.5, cells analyzed
immediately after isolation by sorting; d6.0, reaggregated EBs; d10, cells
from replated EBs. (C) Immunostaining of GFP-Bry+ and
GFP-Bry– cells. Upper panels shows day 2.5 EB sorted cells
stained with an antibody to Foxa2. Positive cells are red/pink in color.
Nuclei of all cells are stained with DAPI (blue). Bottom panels show cells
from 10-day old cultures stained with an antibody to albumin. Positive cells
are indicated by red color. (D) RT-PCR expression analysis of genes associated
with liver maturation in populations derived from GFP-Bry+ and
GFP-Bry– day 2.5 EB cells. Cells from the pre-sorted
population as well as those from the GFP-Bry+ and
GFP-Bry– fractions were reaggregated and cultured in SF
conditions for 8 days. At this stage, the reaggregated EBs were replated into
serum hepatocyte conditions for 4 days, harvested and analyzed.
|
