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Fig. 5. Analysis of kidney capsule grafts of activin-stimulated GFP-Bry+
and GFP-Bry– EB-derived populations. GFP-Bry+ and
GFP-Bry– cells isolated from day 5 EBs differentiated in the
presence of 3 or 100 ng/ml activin were reaggregated and cultured as EBs for
an additional 8 days in SF cultures in the absence of factor. The reaggregated
EBs were replated in hepatocyte conditions for 4 days and then harvested and
transplanted under the kidney capsule of SCID-beige mice. Three weeks
following transplantation, the mice were sacrificed and the kidneys harvested
for analysis. (A) Photograph showing the size of the grafts from the
GFP-Bry+ and GFP-Bry– populations. Size is
indicted by the ruler (mm) at the bottom of the figure. (B) Histological
analysis of grafts from the GFP-Bry+ and GFP-Bry–
populations. SM, skeletal muscle; Gu, gut epithelial-like structure, B, bone;
C, columnar cells; Br, neural tissue; NT, neural tube-like structure.
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