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Fig. 6. Analysis of endoderm derivatives in kidney grafts of GFP-Bry+ populations. (A,B) Immunohistochemistry showing expression of Foxa2 protein in tubular structures present in the grafts. (C,D) Immunohistochemistry demonstrating expression of intestinal fatty acid binding protein (Ifabp) in the gut-like structures present in grafts. (E,F) In situ hybridization indicating expression of surfactant protein C (Sftpc) in the grafts: (E) Bright-field exposure, arrow indicates positive area, (F) dark-field exposure. (G) Hematoxylin and Eosin, and (H) D-PAS staining of consecutive sections demonstrating muscin (M) in the gut epithelial-like structure in the grafts. (I) Skeletal muscle (SM) in a graft from GFP-Bry+ cells induced with 3 ng/ml of activin. K, kidney of recipient.





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