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Fig. 2. Differential encoding of proneural activity in the bHLH domains of NGN proteins and ATO proteins. (A) The basic domains of ATO (red) and NGN1 (purple). Group-specific amino acids are in green. (B) Schematic representation of NGN^bATO with exchanged amino acids in red. (C) Quantitative analysis of proneural activity of misexpressed ATO, NGN1, NGN^bATO in a wild-type background. (Inset) A wing from a uasngn^bato/+; dppGal4/+ fly. (D) Schematic representation of ATO^bNGN with the exchanged amino acids in purple. (E,F) N-tubulin stained Xenopus embryo at stage 19, injected with different mRNAs into one cell (right side) of two cell-stage embryos. (E) 1000 pg of Ato mRNA. (F) 1000 pg of Ato^bNGN mRNA. (G) Schematic representation of NGN^H2ATO and ATO^H2NGN. (H) Quantitative analysis of proneural activity of misexpressed ATO (blue), NGN1 (dark pink), NGN^H2ATO (light pink). (I) N-tubulin stained Xenopus embryo at stage 19, injected with 1000 pg of Ato^H2NGN mRNA into one cell of two-cell stage embryos.

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