QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 3. Mouse NGN1 interacts with DA and Notch, but fails to induce SENS expression. (A) Quantitative analysis of the number of ectopic bristles per fly induced by expressing NGN1 in wild-type, da^+/–, Notch^+/– or E(spl)^+/– background, or co-expression with constitutively active Notch, or the members of E(spl) complex, m8 or m. With the exception of the E(spl)^+/– background, the effects of NGN1 expression in all backgrounds are significantly different from its effects in a wild-type background (P<0.001). (Inset) Autoradiograph of SDS-PAGE gels from co-immunoprecipitation using anti-Myc antibodies of ³⁵S-labeled ATO, MATH1 and NGN1 in the presence and absence of Myc tagged DA. (B) The expression pattern of SENS in cs L3 wing disc, stained with anti-SENS (green). (C) A uasato; dppGal4/+ wing disc, stained with anti-ATO (red) and anti-SENS (green). (D) A uasngn1,dppGal4/TM6 wing disc, stained with ant-NGN1 (red) and anti-SENS (green). (E) A N⁸/+; uasngn1,dppGal4/+ wing disc, stained with anti-NGN1 (red) and anti-SENS (green). (F) Quantitative analysis of the number of SENS positive cells in CS (wild type), uasMath1/+;dppGal4/+, uasngn1,dppGal4/+ and N⁸/+ and N⁸/+;;uasngn1,dppGal4/+ L3 wing discs (n=5).

Return to article