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Fig. 1. The topi meiotic arrest phenotype. (A) Phase-contrast image of
wild-type whole testis, apical tip in top right-hand corner. Primary
spermatocytes occupy most of the apical end (arrows) Elongating spermatid
bundles (arrowheads) are seen inside the testis, and spilling out from the
distal end. (B) Phase-contrast image of topiZ3-2139 whole
testis. Only stages up to mature primary spermatocytes (arrows) are seen.
Degenerating primary spermatocytes are present in the distal region of the
testis (arrowheads). (C-H) High-magnification views of mature spermatocytes
from squashed preparations of wild-type (C-E) and
topiZ3-2139 (F-H) testes viewed by phase-contrast (C,F)
and Hoechst DNA staining (D,G) or both visualization methods simultaneously
(E,H).