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Fig. 3. Antibody stain of Aly and Comr in wild type versus topi. Immunofluorescence staining of Aly (A,D, green in merge) and DNA (B,E, red in merge) on wild-type (D-F) and topiZ3-2139 (A-C) testes revealed that Aly protein is nuclear in both wild type and mutant, but that Aly fails to accumulate on the chromatin in topiZ3-2139 mutant spermatocytes. Immunofluorescence staining of Comr (G,L, green in merge) and DNA (H,M, red in merge) on wild-type (L-N) and topiZ3-2139 (G-I) testes similarly revealed that Comr protein is nuclear in both wild type and mutant, but that Comr fails to accumulate on the chromatin in topiZ3-2139 mutant spermatocytes.





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