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Fig. 4. Molecular cloning of topi. (A) topi genetic region was
defined by the right breakpoints of Df(3R)Gb104 and Df(3R)by10, an interval of
about 60 kb, which contains 23 predicted genes of which five are shown.
topi gene structure and the location of lesions in mutant alleles are
shown. (B) topi predicted amino acid sequence (mel) aligned with the
putative topi orthologues from D. pseudoobsura (pseud) and
A. gambiae (gamb). Sequence identity between all three proteins is
indicated by black boxes, sequence similarity is indicated by grey boxes.
Predicted Zn-finger motifs are indicated with lines above the sequences. The
amino acids affected in the mutant alleles are indicated with an asterisk
above the sequence.
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