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Fig. 7. Differential requirement for topi and achi/vis
in expression of some target genes. (A) RT-PCR of CG8349 from
wild-type and meiotic arrest mutant testes. No-RT is a negative control, gDNA
is PCR on genomic DNA from wild-type flies. Two exposures of the same gel are
shown to emphasize the differences between the mutants and wild type (top),
and the lack of product in achi/vis (bottom). (B-E) In situ
hybridization of CG8349 probe to testes revealed strong expression in
wild-type (B) primary spermatocytes, persisting until mid-late elongation
stages, weaker expression in comr (C) and topi (E), and no
detectable signal in achi/vis (D) testes. (F) RT-PCR of TrxT
from wild-type and meiotic arrest mutant testes. No-RT is a negative control,
gDNA is PCR on genomic DNA from wild-type flies. (G-J) In situ hybridization
of TrxT probe testes revealed strong expression in wild-type (G)
primary spermatocytes and early-mid elongation stages. The signal in
achi/vis testes was of a similar intensity to wild type (I). Weaker
expression was seen in comr (H) and there was no detectable signal in
topi (J) testes.
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