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Fig. 3. Wee2 is required for anterior-posterior embryo elongation, somite
formation, and convergent extension. (A) Wee2-depleted embryos fail to extend
along the anteroposterior axis and fail to form somites. Embryos were treated
as in Fig. 2D, but allowed to
develop until the controls reached stage 25 before being processed for MyoD in
situ analysis. Anterior towards the right, dorsal towards the top. Labels as
in Fig. 1A. Scale bar: 300
µm. (B) Unilateral depletion of Wee2. One blastomere (asterisk) of a
two-cell embryos was microinjected with 40 ng CMO or W2MO.1. These were
allowed to develop until controls reached stage 19 and photographed. Dorsal
view, anterior towards the top. Note curvature and reduced somitic ridge
(arrow) on the Wee2-depleted side. (C) Mesoderm specific gene expression is
unchanged in Wee2-depleted embryos. Quantitative RT-PCR for MyoD, XNot, Vent1,
MA, MHC and ornithine decarboxylase (ODC) from whole, stage 18 embryos treated
with W2MO.1, CMO or nothing (Sibling). (D) Depletion of Wee2 compromises
convergent extension driven elongation of dorsal explants. Embryos were
treated with W2MO.1 or CMO as in Fig.
2D and then processed for dorsal explants. Explants were
photographed when controls reached stage 26. Scale bar: 1 mm.
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