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Fig. 4. Depletion of Wee2 protein disrupts convergent extension of the paraxial
mesoderm during neurulation. (A-C,F) Temporal analysis of Wee2-depleted
embryos during neurulation. Embryos were microinjected as in
Fig. 2D with CMO (odd panels)
or W2MO.1 (even panels), and were allowed to develop until controls reached
the indicated stages before being processed for MyoD (A), XNot (B), MA and MHC
(C), or Sox3 (F) in situ analysis as indicated. Green arrows indicate a closed
blastopore. Lateral limits of paraxial mesoderm (MyoD expression) are
indicated by broken red lines. White and yellow arrows indicate lack of
anterior neural fold and somitic ridge, respectively. Lateral limits of
presumptive neural tissue (Sox3 expression) are indicated by red arrows and
vertical lines. (D) The paraxial mesoderm of Wee2-depleted embryos fails to
converge towards the midline. Representative CMO and W2MO.1 treated, MyoD
stained, stage 18 embryos from 4A were serially sectioned transversely. The
anteroposterior positions of the shown sections are indicated by letters in
right lower corner of panels as per E. Dorsal is towards the top. Black arrow
indicates the forming somitic ridge. (E) Drawing reproduced, with permission,
from Nieuwkoop and Faber (Nieuwkoop and
Faber, 1994) showing position of cuts in D.
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