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Fig. 5. Rescue of Wee2-depleted embryos. Both blastomeres of two-cell embryos were microinjected with a combination of 40 ng W2MO.1 and either injection buffer (panels 1-4), 20 pg Wee2 mRNA (panels 5-8) or 40 pg Wee2 (panels 9-12). These were allowed to develop until non-injected siblings (panels 13-16) reached stage 19. Subsequently, the embryos were subjected to MyoD or Sox3 in situ analysis as indicated. Lateral limits of paraxial mesoderm (MyoD expression) are indicated by broken red lines. Lateral limits of presumptive neural tissue (Sox3 expression) are indicated by red arrows and vertical lines.





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