|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Sequence of the Tribolium Giant ortholog. (A) Alignment of
Tribolium and Drosophila Giant using Clustal W. Identical
and similar amino acids are highlighted in black and grey, respectively.
Longer sequence stretches without homology were omitted from the Dm'Giant
sequence (indicated by dots, the number of omitted amino acids is indicated).
The DNA-binding leucine zipper (large box) is 63% identical and 78% similar
between both insects. The binding domain of the co-repressor CtBP
(small box) is highly conserved, as are a number of additional motifs of
unknown function. These conserved motifs upstream of the leucine zipper
substantiate the orthology of Tc'Giant and Dm'Giant, as they are not present
in other leucine zipper genes. Note that Dm'Giant is twice as large as the
predicted beetle protein. (B) Alignment of the leucine zippers of Tc'Giant,
Dm'Giant, Dm'CG4575 and Hs'HLF, the Human hepatic leukemia factor. Dashes
indicate sequence identity. The position of the three nested PCR primers used
for isolation of Tc'giant is given below.
|
