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Fig. 3. Double in situ hybridization with Tc'giant in brown and
Tc'eve (A-D), Tc'hairy (E) and Tc'Krüppel
(F-H) in blue. eve stripes are numbered, with a/b, indicating the
secondary segmental stripes derived from the respective double-segmental
primary stripe. (A-D) The Tc'giant stripes in maxilla, T3 and A2
coincide roughly with the first, third and fourth Tc'eve stripes,
respectively, and mature in the same relation to each other: early
Tc'eve stripes are shifted slightly anterior to the giant
stripes. As the pattern matures, the stripes successively coincide (see stripe
3 in B and C). (E) Tc'hairy is expressed in a frame roughly
complementary to Tc'eve. Its posterior expression borders initially
overlap the Tc'giant stripes but the overlap fades with time and the
borders eventually abut each other. The anterior borders of the hairy
stripes always remain separated from Tc'giant stripes. (F)
Tc'Krüppel appears at the posterior pole of the blastoderm
within giant free tissue (compare with C in
Fig. 2). (G,H) The posterior
Tc'giant domain-arises right within the Tc'Krüppel
domain and the genes remain co-expressed in the third thoracic segment (T3 in
H). Strong mutual repression as described for the Drosophila
orthologs seems unlikely for this region. In addition, this staining shows
that Tc'Krüppel is not expressed posteriorly to T3.
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