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Fig. 1. Comparison of the amino acid sequence of fish Charon with the sequences of
other Cerberus/Dan family members. (A) Alignment was done with the ClustalW
program. Identities in the amino acid sequence of zebrafish (z) Charon to the
sequences Fugu Charon, flounder (fl) Charon, Xenopus (X)
Cerberus, chick (c) Caronte, and human Cerberus-related (hCerberus) are 41%,
41%, 18%, 25%, 20%, respectively. Fugu Charon and flounder Charon are
the most similar to each other (63%). (B) A radial phylogenic tree of the
Cerberus/Dan family proteins. The tree was calculated according to the
Expansion of the ClustalW program by DDBJ (DDBJ;
http://www.ddbj.nig.ac.jp)
using the amino acid sequences of the cysteine-knot domain of the proteins.
Zebrafish PRDC and zebrafish Gremlin are less similar (16% and 18%,
respectively) to chick Caronte than to zebrafish Charon. c, chick; X,
Xenopus; z, zebrafish.
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