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Fig. 2. charon expression in zebrafish. In situ hybridization analysis
revealed that charon expression was initiated around 12 hpf at the
6-somite stage in the tailbud (A,B). charon expression became more
obvious at 14 hpf (10-somite stage) (C,D). The charon transcripts
were observed in the posterior half of the flanking domain of Kupffer's
vesicle in a horseshoe shape (D, refer to E,F). The expression continued
through 15 hpf to 18 hpf in the same tissue (16 hpf, 14-somite stage, G,H).
The expression pattern of charon mRNA in Fugu (I,J) and
flounder (K,L, refer to M) was very similar to that in zebrafish. The
charon transcripts were not detected in any other domain throughout
embryogenesis. There was no obvious L/R bias in the strength of
charon expression in the majority of embryos. (A,C,E,G,I,K,M) Lateral
views. (B,D,F,H,J,L) Vegetal pole views of the tailbud region. (E,F,M) Control
non-stained embryos. Arrowheads indicate the position of Kupffer's
vesicle.
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