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Fig. 3. Regulation of charon expression. Expression of charon in
the wild-type (A), mutant (B-H) and antivin/lefty1 RNA-injected
(atv inj.) embryos (I) at the 10-somite stage (13 hpf). Vegetal pole
views of the tailbud region. In bozozok (boz) embryos,
charon expression was reduced (C) or absent (B). In no tail
(ntl) embryos, no charon expression was detected (D). In
squint (sqt) embryos, charon expression was reduced
(F) or absent (E). In cyclops (cyc) embryos, charon
expression was not affected (G). In one-eyed pinhead (oep)
embryos, the strength of charon expression was not affected, but the
expression domain became smaller, in proportion to the size of Kupffer's
vesicle (G). In embryos injected with 25 pg of antivin/lefty1 RNA,
which displayed phenotypes similar to cyc:sqt double mutant and
maternal-zygotic oep mutant embryos at 24 hpf (data not shown), no
charon expression was detected (I). Variability of charon
expression in boz and sqt embryos was consistent with the
variable expressivity of these boz and sqt alleles.
Arrowheads indicate weak expression of charon.
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